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V e h i c l e 2 0 μ M i n h i b i t o r D M S O 1 μ M i n h i b i t o r 2 μ M i n h i b i t o r 1 0 μ M i n h i b i t o r 2 0 μ M i n h i b i t o r -0.2 0.0 0.2 0.4 0.6 0.8 1.0 1.2 Bleb Frequency S7 Fig S7 Figure. Inhibition of bleb formation by necroptosis inhibitor GWX806742X. The graph represents data from two experiments with cell counts from n≥6 fields of view (n>80 cells per treatment group). HCLE cells treated with GWX 806742X were challenged with wild-type S. marcescens strain K904 at MOI=50 and after 2 h cells were imaged and bleb frequency was measured. Mean and SD are shown. ANOVA with Tukey's post-test was used and significance is shown by asterisks. \* p<0.05, \*\* p<0.01, \*\*\*\* p<0.0001. Data suggests specific inhibition of necroptosis mediator MLKL reduces bleb formation. S. marcescens (MOI 50) \*\*\*\* \* \*\*

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Publisher

Public Library of Science (PLoS)

Language

EN

Field

Medicine (Health Sciences)