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Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport by Faten AbdelHafez; Jing Xu; Jeffrey Goldberg; Nina Desai is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background High cooling rates with vitrification can be achieved through the use of carriers that allow cryopreservation in fluid volumes < one μl. Open carriers allow direct contact of embryos with liquid nitrogen (LN2) whereas closed carrier systems sequester the embryo within a sealed system during immersion in LN2. The use of closed systems may be preferable to reduce the possibility of cross-contamination. In the present study, we compare open and closed carriers for vitrification of embryos. We also examine their ability to retain embryo viability during vapor phase transport. ## Methods Frozen one-cell mouse embryos were thawed and randomly allocated to treatment groups. Embryos were cultured and vitrified at the 8-cell (CL) or at the blastocyst (BL) stage. The cryoloop, an open carrier was tested against two closed systems, the Cryotip and the HSV straw. Carriers were tested for their ability to maintain embryo viability when held in the vapor phase of a dry shipper for a period of 96 hours. Outcome parameters monitored were embryo survival, recovery, subsequent development and signs of DNA damage. ## Results A total of 561 embryos were vitrified. The only parameter sign

Who reads Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport?

Author

Faten AbdelHafez; Jing Xu; Jeffrey Goldberg; Nina Desai

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6750)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)