About this Immunology and Microbiology article
HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry by Michael Roche; Martin R Jakobsen; Anne Ellett; Hamid Salimiseyedabad; Becky Jubb; Mike Westby; Benhur Lee; Sharon R Lewin; Melissa J Churchill; Paul R Gorry is a Immunology and Microbiology article available to read on EtoBox.
## Background Maraviroc (MVC) and other CCR5 antagonists are HIV-1 entry inhibitors that bind to- and alter the conformation of CCR5, such that CCR5 is no longer recognized by the viral gp120 envelope (Env) glycoproteins. Resistance to CCR5 antagonists results from HIV-1 Env acquiring the ability to utilize the drug-bound conformation of CCR5. Selecting for HIV-1 resistance to CCR5-antagonists __in vitro__ is relatively difficult. However, the CCR5-using CC1/85 strain appears to be uniquely predisposed to acquiring resistance to several CCR5 antagonists __in vitro__ including MVC, vicriviroc and AD101. ## Findings Here, we show that Env derived from the parental CC1/85 strain is inherently capable of a low affinity interaction with MVC-bound CCR5. However, this phenotype was only revealed in 293-Affinofile cells and NP2-CD4/CCR5 cells that express very high levels of CCR5, and was masked in TZM-bl, JC53 and U87-CD4/CCR5 cells as well as PBMC, which express comparatively lower levels of CCR5 and which are more commonly used to detect resistance to CCR5 antagonists. ## Conclusions Env derived from the CC1/85 strain of HIV-1 is inherently capable of a low-affinity interaction with MVC
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Michael Roche; Martin R Jakobsen; Anne Ellett; Hamid Salimiseyedabad; Becky Jubb; Mike Westby; Benhur Lee; Sharon R Lewin; Melissa J Churchill; Paul R Gorry
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, c2004-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1742-4690)
- Published
- 2011
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)