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The Donnan phenomenon describes the unequal distribution of diffusible ions across a dialysis membrane due to the presence of a non-diffusible macromolecule, leading to Donnan equilibrium. The document outlines experimental approaches to study this phenomenon, theoretical underpinnings involving electrochemical potentials, and the impact of pH on protein charge. It concludes that the plasma membrane functions similarly to a dialysis membrane, influencing electrolyte composition between plasma and interstiti

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