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Ultra-rare-event detection performance of a custom scanning cytometer on a model preparation of fetal nRBCs by Shruti Bajaj; John B. Welsh; Robert C. Leif; Jeffrey H. Price is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Ultra-rare-event detection performance of a custom scanning cytometer on a model preparation of fetal nRBCs about?

## Background: The performance of a fully automated scanning cytometer incorporating previously reported highprecision autofocus and accurate image segmentation was evaluated for the detection of "ultra-rare" cells using a model of fetal nucleated red blood cells (fnRBCs) in the maternal circulation. These distinctive scanning cytometry techniques were expected to markedly improve sensitivity and specificity. Methods: Normal adult blood and fetal red blood cells were stained with fluorescein isothiocyanate-conjugated anti-fetal hemoglobin and 4,6-diamidino-2-phenylindole, a nuclear dye. Adult cells were spiked with fetal cells to create ratios of about 1 fnRBC in 10 7 nucleated cells and deposited in monolayers on slides using centrifugal cytology. Rare-event performance parameters were reviewed, formalized, and applied to test the new instrument using this cell model. Results: Fifteen slides were analyzed to establish performance by comparison with manual detection, and four sets of four slides each were then scanned to explore the limit of detection. Results were an average sensitivity of 91%, an average specificity error of 12.3 false-positives per million cells, and repeatabili

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Author

Shruti Bajaj; John B. Welsh; Robert C. Leif; Jeffrey H. Price

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)