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Ligand Preference and Orientation in B- and C-type Heme-binding Proteins by Christian Fufezan; Jun Zhang; M. R. Gunner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Ligand Preference and Orientation in B- and C-type Heme-binding Proteins about?

## Abstract Hemes are often incorporated into designed proteins. The importance of the heme ligand type and its orientation is still a matter of debate. Here, heme ligands and ligand orientation were investigated using a nonredundant (87 structures) and a redundant (1503 structures) set of structures to compare and contrast design features of natural __b__‐ and __c__‐type heme‐binding proteins. Histidine is the most common ligand. Marked differences in ligation motifs between __b__‐ and __c__‐type hemes are higher occurrence of His‐Met in __c__‐type heme binding motifs (16.4% vs. 1.4%) and higher occurrence of exchangeable, small molecules in __b__‐type heme binding motifs (67.6% vs. 9.9%). Histidine ligands that are part of the __c__‐type CXXCH heme‐binding motif show a distinct asymmetric distribution of orientation. They tend to point between either the heme propionates or between the NA and NB heme nitrogens. Molecular mechanics calculations show that this asymmetry is due to the bonded constraints of the covalent attachment between the heme and the protein. In contrast, the orientations of __b__‐type hemes histidine ligands are found evenly distributed with no preference. Obse

Who reads Ligand Preference and Orientation in B- and C-type Heme-binding Proteins?

Author

Christian Fufezan; Jun Zhang; M. R. Gunner

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)