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Stimulation of Cartilage-matrix Proteoglycan Synthesis by Morphologically Transformed Chondrocytes Grown in the Presence of Fibroblast Growth Factor and Transforming Growth Factor-beta by Hiroyuki Inoue; Yukio Kato; Masahiro Iwamoto; Yuji Hiraki; Mamoru Sakuda; Fujio Suzuki is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Stimulation of Cartilage-matrix Proteoglycan Synthesis by Morphologically Transformed Chondrocytes Grown in the Presence of Fibroblast Growth Factor and Transforming Growth Factor-beta about?
The effects of transforming growth factor-beta (TGF-beta) on the synthesis of carii lage-r~ki~ rix protenglycan by cultured rabbit chondrocytes were examined. Rabbit chondrocytes were seeded at low density and exposed to a 1 :1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12 medium supplemented with 0.5% fetal bovine serum, 1 % bovine serum albumin, 50 pdml ascorbic acid, and 2 x lop7 M hydrocortisone (Medium A). Various combinations of TGF-beta, insulin-like growth factor-I (IGF-I), and fibroblast growth factor (FGF) were also added to Medium A, and the chondrocytes were grown to confluency. Chondrocytes grown with TGF-beta or FGF alone became flat or libroblastic, those grown with FCF and TGF-beta became very elongated and formed distinct foci, and those grown with FCF and IGF-I showed the spherical configuration characteristic of overtly differentiated chondrocytes. Nevertheless, the incorporation of 'H with glucosamine into the large, chondroitin sulfate proteoglycan synthesized by cultures with FGF and TCF-beta was similar to that in cells grown with F C F and IGF-I and five times that in cells cultured with FCF alone. The increases in incorporation of 'H reflecte
Who reads Stimulation of Cartilage-matrix Proteoglycan Synthesis by Morphologically Transformed Chondrocytes Grown in the Presence of Fibroblast Growth Factor and Transforming Growth Factor-beta?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hiroyuki Inoue; Yukio Kato; Masahiro Iwamoto; Yuji Hiraki; Mamoru Sakuda; Fujio Suzuki
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 1989
- Language
- EN
- ISBN
- 9781041380214
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)