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Segregation of Micron‐scale Membrane Sub‐domains in Live Murine Sperm by Vimal Selvaraj; Atsushi Asano; Danielle E. Buttke; John L. McElwee; Jacquelyn L. Nelson; Collin A. Wolff; Tanya Merdiushev; Miguel W. Fornés; Alex W. Cohen; Michael P. Lisanti; George H. Rothblat; Gregory S. Kopf; Alexander J. Travis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Segregation of Micron‐scale Membrane Sub‐domains in Live Murine Sperm about?

## Abstract Lipid rafts, membrane sub‐domains enriched in sterols and sphingolipids, are controversial because demonstrations of rafts have often utilized fixed cells. We showed in living sperm that the ganglioside G~M1~ localized to a micron‐scale membrane sub‐domain in the plasma membrane overlying the acrosome. We investigated four models proposed for membrane sub‐domain maintenance. G~M1~ segregation was maintained in live sperm incubated under non‐capacitating conditions, and after sterol efflux, a membrane alteration necessary for capacitation. The complete lack of G~M1~ diffusion to the post‐acrosomal plasma membrane (PAPM) in live cells argued against the transient confinement zone model. However, within seconds after cessation of sperm motility, G~M1~ dramatically redistributed several microns from the acrosomal sub‐domain to the post‐acrosomal, non‐raft sub‐domain. This redistribution was not accompanied by movement of sterols, and was induced by the pentameric cholera toxin subunit B (CTB). These data argued against a lipid–lipid interaction model for sub‐domain maintenance. Although impossible to rule out a lipid shell model definitively, mice lacking caveolin‐1 maintai

Who reads Segregation of Micron‐scale Membrane Sub‐domains in Live Murine Sperm?

Author

Vimal Selvaraj; Atsushi Asano; Danielle E. Buttke; John L. McElwee; Jacquelyn L. Nelson; Collin A. Wolff; Tanya Merdiushev; Miguel W. Fornés; Alex W. Cohen; Michael P. Lisanti; George H. Rothblat; Gregory S. Kopf; Alexander J. Travis

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)