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Transcriptional Repression of the Microphthalmia Gene in Melanoma Cells Correlates with the Unresponsiveness of Target Genes to Ectopic Microphthalmia-Associated Transcription Factor by Vachtenheim, J.; Novotna, H.; Ghanem, G. is a Medicine article available to read on EtoBox.

What is Transcriptional Repression of the Microphthalmia Gene in Melanoma Cells Correlates with the Unresponsiveness of Target Genes to Ectopic Microphthalmia-Associated Transcription Factor about?

In the melanocyte, expression of genes required for pigment formation is mediated by the microphthalmia transcription factor, which is also critical for the development and survival of normal melanocytes during embryogenesis. Here we show that the expression of the melanocyte-specific isoform of microphthalmia transcription factor is lost in a subset of human melanoma cell lines, accompanied by the repression of tyrosinase and tyrosinase-related proteins 1 and 2, the three transcriptional target genes for microphthalmia. After the forced expression of microphthalmia transcription factor in melanoma cells where the expression of endogenous microphthalmia gene was found to be extinguished, no restoration of the melanogenic phenotype occurred and the transcription of the three microphthalmia transcription factor target genes remained silent. The transcription activation domain of microphthalmia transcription factor, tested as a GAL-MITF fusion protein, remained fully functional in these cells, however, and ectopic microphthalmia transcription factor localized normally to the nucleus and bound to the tyrosinase initiator E-box in gel retardation assays. Thus, the block of differentiati

Who reads Transcriptional Repression of the Microphthalmia Gene in Melanoma Cells Correlates with the Unresponsiveness of Target Genes to Ectopic Microphthalmia-Associated Transcription Factor?

Author

Vachtenheim, J.; Novotna, H.; Ghanem, G.

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; Springer Science and Business Media LLC; Wiley (ISSN 0022-202X)

Published

2002

Language

EN

Field

Medicine (Health Sciences)