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Molecular biology analysis of ABO blood group variants caused by natural chimaerism by Yuqing Wang; Qiuju Mou; Hang Lei; Hasiyati Heililahong; Wei Zou; Xuefeng Wang; Chengrui Qian; Xiaohong Cai is a Medicine article available to read on EtoBox.

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## Abstract ## Background and Objectives The chimaerism phenomenon constitutes a significant mechanism underlying ABO phenotype discrepancies; however, its detection has technical challenges. In the current study, we explored different techniques to establish the chimaeric status of ABO blood types. ## Materials and Methods Fifteen individuals with possible chimaeric ABO blood type, as suggested by standard tube or column agglutination method and RBC adsorption‐elution test, were enrolled in the study. The red blood cells from 11 investigated subjects showed mix‐field agglutination with anti‐A or anti‐B in blood typing; weak A or B antigens on the other four individuals' RBCs were detected by adsorption‐elution tests. The genetic study was conducted with PCR‐SSP genotype, DNA sequencing of the ABO gene, STR analysis and ddPCR. ## Results The genetic chimaeric status was confirmed in four (27%) individuals by SSP test alone. The ABO gene sequencing identified an additional ABO allele and enabled chimaerism detection in 10 (67%) subjects. The STR analyses established the chimaerism status in 13 (87%) individuals. In the two cases where neither of the tests mentioned above had positiv

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Author

Yuqing Wang; Qiuju Mou; Hang Lei; Hasiyati Heililahong; Wei Zou; Xuefeng Wang; Chengrui Qian; Xiaohong Cai

Publisher

Wiley

Published

2022

Language

EN

Field

Medicine (Health Sciences)