Opening book details…
Can I read Quantitation of sorafenib and its active metabolite sorafenib N-oxide in human plasma by liquid chromatography–tandem mass spectrometry on EtoBox?
Quantitation of sorafenib and its active metabolite sorafenib N-oxide in human plasma by liquid chromatography–tandem mass spectrometry by Lie Li; Ming Zhao; Fariba Navid; Keith Pratz; B. Doug Smith; Michelle A. Rudek; Sharyn D. Baker is a Chemistry article available to read on EtoBox.
What is Quantitation of sorafenib and its active metabolite sorafenib N-oxide in human plasma by liquid chromatography–tandem mass spectrometry about?
A simple and rapid method with high performance liquid chromatography/tandem mass spectrometry is described for the quantitation of the kinase inhibitor sorafenib and its active metabolite sorafenib N-oxide in human plasma. A protein precipitation extraction procedure was applied to 50 μL of plasma. Chromatographic separation of the two analytes, and the internal standard [(2)H(3)(13)C]-sorafenib, was achieved on a C(18) analytical column and isocratic flow at 0.3 mL/min for 4 min. Mean within-run and between-run precision for all analytes were <6.9% and accuracy was <5.3%. Calibration curves were linear over the concentration range of 50-10,000 ng/mL for sorafenib and 10-2500 ng/mL for sorafenib N-oxide. This method allows a specific, sensitive, and reliable determination of the kinase inhibitor sorafenib and its active metabolite sorafenib N-oxide in human plasma in a single analytical run.
Who reads Quantitation of sorafenib and its active metabolite sorafenib N-oxide in human plasma by liquid chromatography–tandem mass spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Lie Li; Ming Zhao; Fariba Navid; Keith Pratz; B. Doug Smith; Michelle A. Rudek; Sharyn D. Baker
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2010
- Language
- EN
- Field
- Chemistry (Physical Sciences)