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Genome-wide Identification of DNA-protein Interaction to Reconstruct Bacterial Transcription Regulatory Network by Joon Young Park; Hemraj Rimal; Ina Bang; Linh Khanh Nong; Donghyuk Kim is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Genome-wide Identification of DNA-protein Interaction to Reconstruct Bacterial Transcription Regulatory Network about?

The development and innovative use of nextgeneration sequencing technologies have opened the doors for genetic and epigenetic research at the next level. These technologies can be used to study gene expression regulation at the transcriptional and post-transcriptional levels in both prokaryotic and eukaryotic systems. In this review, we focused on the various tools and techniques for DNA-binding proteins such as RNA polymerase, sigma factors, nucleoidassociated proteins, and transcription factors in bacteria. Descriptions on series of Chromatin ImmunoPrecipitation (ChIP) technologies, including ChIP followed by microarray hybridization (ChIP-chip), ChIP followed by deep sequencing (ChIP-seq), and ChIP with exonuclease digestion and deep sequencing (ChIP-exo) has been given. Furthermore, recent investigations on transcriptional regulatory networks of a wide range of bacteria with ChIP technologies are discussed for the model bacteria Escherichia coli followed by pathogenic and other non-pathogenic bacteria. In conclusion, ChIP technologies have proven effective and efficient to reconstruct and to delineate transcriptional regulatory network in a variety of bacteria.

Who reads Genome-wide Identification of DNA-protein Interaction to Reconstruct Bacterial Transcription Regulatory Network?

Author

Joon Young Park; Hemraj Rimal; Ina Bang; Linh Khanh Nong; Donghyuk Kim

Publisher

Springer Science and Business Media LLC

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)