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What is Sanger Sequencing: Chain Termination Method about?

Sanger sequencing is a method for determining the sequence of nucleotides in a piece of DNA. It involves making copies of a DNA fragment that end randomly at each nucleotide position due to the incorporation of chain-terminating dideoxy nucleotides labeled with different dyes. The fragments of different lengths are then separated by gel electrophoresis and detected by a laser, allowing the sequence to be read out from the order of fluorescent dye colors. While Sanger sequencing provides high-quality sequenc

Author

ansah

Language

EN