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DNA fragmentation during bone formation in neonatal rodents assessed by transferase-mediated end labeling by A. L. J. J. Bronckers; W. Goei; G. Luo; G. Karsenty; R. N. D'Souza; D. M. Lyaruu; E. H. Burger is a Medicine article available to read on EtoBox.

What is DNA fragmentation during bone formation in neonatal rodents assessed by transferase-mediated end labeling about?

To study the fate of bone cells, we used the transferase-mediated, biotin-dUTP nick end-labeling (TUNEL) assay to detect DNA fragmentation during the formation of intramembranous and endochondral bone in newly born hamsters, mice, and rats. In alveolar bone forming around the developing tooth crowns, DNA fragmentation was found in three cell types: =-negative mononuclear cells at the bone surface, osteocytes, and some but not all nuclei of TRAP-positive osteoclasts. Osteoblasts did not undergo DNA fragmentation. A strong positive correlation was found between contacts of TUNEL-positive osteocytes and osteoclasts. Extracellular bone matrix also stained occasionally for the presence of DNA fragments. During endochondral bone formation, TUNEL staining was detected in late hypertrophic chondrocytes of the epiphyseal growth plate. During rapid longitudinal growth of long bones, TUNEL-positive hypertrophic chondrocytes were found coincident with or slightly after invasion of blood vessels from the diaphysis. However, during slow longitudinal growth and in secondary ossification centers, DNA fragmentation was seen in hypertrophic chondrocytes still located within their lacunae. We conclud

Who reads DNA fragmentation during bone formation in neonatal rodents assessed by transferase-mediated end labeling?

Author

A. L. J. J. Bronckers; W. Goei; G. Luo; G. Karsenty; R. N. D'Souza; D. M. Lyaruu; E. H. Burger

Publisher

American Society for Bone and Mineral Research; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0884-0431)

Published

1996

Language

EN

ISBN

9785650110910

Field

Medicine (Health Sciences)