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Genetic characterization of Cryptosporidium parvum from calves by mutation scanning and targeted sequencing – zoonotic implications by Matthew J. Nolan; Aaron R. Jex; Peter D. Mansell; Glenn F. Browning; Robin B. Gasser is a Engineering article available to read on EtoBox.

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## Abstract This study explored the genetic make‐up of __Cryptosporidium__ in fecal samples from 268 individual calves on pasture‐based dairy farms in three regions of Victoria, Australia. An integrated approach, using PCR‐coupled single‐strand conformation polymorphism, targeted sequencing and phylogenetic analysis, was employed to classify the genetic variants (__i.e__. genotypes and subgenotypes) of __Cryptosporidium parvum__ present in 124 (46.3%) samples and to infer their zoonotic potential. Genotypic and subgenotypic classification was achieved using a portion of the 60 kDa glycoprotein gene (designated p__gp60__); specific identity was verified using a region within the small subunit of the nuclear ribosomal RNA (pSSU). Twelve sequence types representing ten distinct subgenotypes were defined within genotype IIa, namely IIaA16G3R1 (__n__=7), IIaA17G2R1 (1), IIaA18G2R1a (2), IIaA18G2R1b (1), IIaA18G4R1 (1), IIaA19G3R1a (80), IIaA19G3R1b (1), IIaA20G2R1 (9), IIaA20G3R1 (1), IIaA20G4R1 (9), IIaA21G3R1 (1) and IIaA23G3R1 (9), of which IIaA18G2R1b, IIaA18G4R1 and IIaA19G3R1b are new records. All of the subgenotypes, except IIaA16G3R1, IIaA18G4R1 and IIaA20G4R1, have been detecte

Who reads Genetic characterization of Cryptosporidium parvum from calves by mutation scanning and targeted sequencing – zoonotic implications?

Author

Matthew J. Nolan; Aaron R. Jex; Peter D. Mansell; Glenn F. Browning; Robin B. Gasser

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)

Published

2009

Language

EN

Field

Engineering (Physical Sciences)