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Neonatal exposure of bisphenol A led to increased lipolysis of visceral adipose tissue in adult rats with DNA hypomethylati ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Animal care and experimental design 2 2.2 Blood and adipose tissue sampling 2 2.3 Biochemical assays 2 2.4 Histology 2 2.5 3T3-L1 cell line 3 2.6 RNA and DNA extraction 3 2.7 RT-qPCR analysis 3 2.8 CT conversion and mass array EpiTYPER quantitative DNA methylation analysis 3 2.9 Statistical analysis 3 3 Results 3 3.1 Neonatal exposure of BPA up-regulated the levels of serum FFA, glycerol and glucose in adult male rats after fasting fo ... 3 3.2 Neonatal exposure of BPA increased levels of FFA and glycerol in visceral adipose tissue of adult male rats, without af ... 3 3.3 Neonatal exposure of BPA did not affect the transcription levels of lipogenic genes in visceral adipose tissue of adult ... 3 3.4 Neonatal exposure of BPA resulted in increased expression of lipolytic genes in visceral adipose tissue of adult male r ... 4 3.5 BPA administration throughout the differentiation stage of 3T3-L1 cell led to decreased level of DNA methylation and in ... 5 4 Discussion 5 5 Conclusions 7 Fundings 7 CRediT authorshi

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