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Serum deprivation and NGF induce and modulate voltage‐gated Na^+^ currents in human astrocytoma cell lines by Robert Kraft; Daniel Basrai; Klaus Benndorf; Stephan Patt is a Neuroscience article available to read on EtoBox.

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## Abstract Glial tumor cells derived from primary tissue express large voltage‐gated Na^+^ currents, whereas glioma cell lines usually lack this feature. We studied the effect of serum deprivation on the expression of Na^+^ currents in two astrocytoma cell lines (1321N1 and A172). Serum deprivation for more than 2 days sufficed to induce large Na^+^ currents in both cell lines; 300 nM of the specific blocker of voltage‐gated Na^+^ channels, tetrodotoxin, blocked these currents by about 85%. During serum deprivation, the cells also underwent morphological changes that were characterized by cell rounding and outgrowth of processes. Treatment with 100 ng/ml nerve growth factor (NGF) promoted these morphological changes and also accelerated the development of Na^+^ currents. In 1321N1 cells, NGF increased the Na^+^ current density after short serum deprivation (3–6 d) and changed several gating properties after longer serum deprivation (9–13 d). In comparison with cells from the early culture stage (3–6 d), the steady‐state inactivation of the Na^+^ current was shifted by −24 mV in NGF‐treated cells from the late (9–13 d) culture stage. In untreated cells, this shift was only −13 mV.

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Author

Robert Kraft; Daniel Basrai; Klaus Benndorf; Stephan Patt

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2001

Language

EN

Field

Neuroscience (Life Sciences)