About this Medicine article
Generation of human osteoclasts in stromal cell‐free and stromal cell‐rich cultures: differences in osteoclast CD11c/CD18 integrin expression by Charlotte S. Lader; John Scopes; Michael A. Horton; Adrienne M. Flanagan is a Medicine article available to read on EtoBox.
Osteoclasts form in the presence of macrophage colony‐stimulating factor (M‐CSF) and receptor activator of Nfκb ligand (RANKL), a membrane‐bound differentiation factor that is now available as a soluble recombinant molecule. Acquisition of the osteoclast phenotype [the α~v~β~3~ subunit of the vitronectin receptor (VNR)‐, calcitonin receptor (CTR)‐ and F‐actin ring‐positive cells] is associated with loss of monocyte/macrophage‐associated integrins, specifically CD11b, CD11c and CD18. We hypothesized that differences in the osteoclast integrin adhesion molecule profile may exist in osteoclasts generated in stromal cell‐rich and in stromal‐free conditions. Unlike osteoclasts generated __in vivo__, F‐actin ring‐positive (resorbing) osteoclasts formed in soluble RANKL __in vitro__, in the absence of stromal cells, and co‐expressed CD11c and CD18. However, when osteoclasts were generated from peripheral blood mononuclear cells (PBMNCs) in co‐cultures with the murine bone marrow stromal cell line 218 (which does not produce membrane‐bound RANKL) in the presence of soluble RANKL, CD11c and CD18 were not expressed by osteoclasts. These findings indicate that the persistent expression of CD1
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- Author
- Charlotte S. Lader; John Scopes; Michael A. Horton; Adrienne M. Flanagan
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)
- Published
- 2001
- Language
- EN
- Field
- Medicine (Health Sciences)