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Data mining crystallization databases: Knowledge-based approaches to optimize protein crystal screens by Matthew S. Kimber; François Vallee; Simon Houston; Alexander Nečakov; Tatiana Skarina; Elena Evdokimova; Steven Beasley; Dinesh Christendat; Alexei Savchenko; Cheryl H. Arrowsmith; Masoud Vedadi; Mark Gerstein; Aled M. Edwards is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Data mining crystallization databases: Knowledge-based approaches to optimize protein crystal screens about?
## Abstract Protein crystallization is a major bottleneck in protein X‐ray crystallography, the workhorse of most structural proteomics projects. Because the principles that govern protein crystallization are too poorly understood to allow them to be used in a strongly predictive sense, the most common crystallization strategy entails screening a wide variety of solution conditions to identify the small subset that will support crystal nucleation and growth. We tested the hypothesis that more efficient crystallization strategies could be formulated by extracting useful patterns and correlations from the large data sets of crystallization trials created in structural proteomics projects. A database of crystallization conditions was constructed for 755 different proteins purified and crystallized under uniform conditions. Forty‐five percent of the proteins formed crystals. Data mining identified the conditions that crystallize the most proteins, revealed that many conditions are highly correlated in their behavior, and showed that the crystallization success rate is markedly dependent on the organism from which proteins derive. Of the proteins that crystallized in a 48‐condition expe
Who reads Data mining crystallization databases: Knowledge-based approaches to optimize protein crystal screens?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Matthew S. Kimber; François Vallee; Simon Houston; Alexander Nečakov; Tatiana Skarina; Elena Evdokimova; Steven Beasley; Dinesh Christendat; Alexei Savchenko; Cheryl H. Arrowsmith; Masoud Vedadi; Mark Gerstein; Aled M. Edwards
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)