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Can I read localisation of non-replicating heterochromatin in polytene cells of Drosophila nasuta by fluorescence microscopy on EtoBox?
localisation of non-replicating heterochromatin in polytene cells of Drosophila nasuta by fluorescence microscopy by Mahesh Kumar; S. C. Lakhotia is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is localisation of non-replicating heterochromatin in polytene cells of Drosophila nasuta by fluorescence microscopy about?
A simple fluorescence technique is decribed to localise in situ the non-replicating alpha heterochromatin in the chromocentre region of Drosophila nasuta polytene nuclei. After incorporating 5-bromodeoxyuridine in larval salivary gland cells for one or two cycles of replication, the polytene nuclei are examined for Hoechst 33258 flourescence at pH 7.0. The non-replicating alpha heterochromatin remains brightly fluorescing as it does not incorporate any 5-bromodeoxyuridine while the rest of the replicating chromatin shows dull fluorescence due to the quenching of Hoechst 33258 fluorescence by the bromodeoxyuridine substituted DNA.
Who reads localisation of non-replicating heterochromatin in polytene cells of Drosophila nasuta by fluorescence microscopy?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mahesh Kumar; S. C. Lakhotia
- Publisher
- Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0009-5915)
- Published
- 1977
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)