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Ovariectomy induces abdominal fat accumulation by improving gonadotropin-releasing hormone secretion in mouse 1 1. Introduction 1 2. Materials and methods 2 2.1. Ethics statement 2 2.2. Animals and treatment 2 2.3. Sample collection 2 2.4. Radioimmunoassay (RIA) 2 2.5. Immunohistochemistry (IHC) 2 2.6. Cell culture and treatment 2 2.7. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay 2 2.8. Oil Red O staining assay 2 2.9. Flow cytometry (FCM) assay 2 2.10. Quantitative polymerase chain reaction (Q-PCR) 2 2.11. Western blotting 2 2.12. Intracellular protein kinase A (PKA) assay 2 2.13. Statistical analysis 3 3. Results 3 3.1. Ovariectomy increases abdominal fat accumulation and changes the gonad axis hormone levels in blood of mouse 3 3.2. GnRH located in abdominal fat tissue in mouse 3 3.3. GnRH promotes cell proliferation, but no direct effect on differentiation of 3T3-L1 cells 3 3.4. GnRHR mediates the effect of GnRH via PKA-CREB pathway in abdominal fat tissue 3 3.5. GnRH induces the secretion of FSH to accelerate adipocyte differentiation 3 4. Discussion 4 Declaration of competing interest 7 Acknowledgements 7 References 7

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UND

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nonfiction