Opening book details…
Can I read Complete Circular Genome Sequence of a Multidrug-Resistant Escherichia coli Strain from Cuba Obtained with Nanopore and Illumina Hybrid Assembly on EtoBox?
Complete Circular Genome Sequence of a Multidrug-Resistant Escherichia coli Strain from Cuba Obtained with Nanopore and Illumina Hybrid Assembly by Hernández-Fillor, Rosa Elena; Brilhante, Michael; Espinosa, Ivette; Perreten, Vincent; Rasko, David is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Complete Circular Genome Sequence of a Multidrug-Resistant Escherichia coli Strain from Cuba Obtained with Nanopore and Illumina Hybrid Assembly about?
The complete genome sequence of a multidrug-resistant Escherichia coli strain isolated from a healthy pig in Cuba was determined using short and long reads. This strain carried four plasmids, including a 42,683-kb IncX1 plasmid, which contains the third-generation cephalosporin resistance gene bla CTX-M-32 together with other disinfectant and antibiotic resistance genes. C ommensal Escherichia coli from animals represents a reservoir of acquired antibiotic resistance elements, which can be transferred to pathogenic E. coli strains (1). The multidrug-resistant (MDR) E. coli strain described here is part of a larger study screening pigs for the presence of third-generation cephalosporin-resistant E. coli in Cuba, where bla CTX-M-32 was found to be a predominant third-generation cephalosporin resistance gene. We completely sequenced one of these CTX-M-32-containing E. coli strains with both short and long reads to gain information on the structure of the mobile genetic elements and localization of the antibiotic resistance genes. A rectal swab from a healthy pig was collected in a swine facility of the Matanzas municipality in July 2016 and was grown overnight at 37°C on a MacConkey (
Who reads Complete Circular Genome Sequence of a Multidrug-Resistant Escherichia coli Strain from Cuba Obtained with Nanopore and Illumina Hybrid Assembly?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hernández-Fillor, Rosa Elena; Brilhante, Michael; Espinosa, Ivette; Perreten, Vincent; Rasko, David
- Publisher
- American Society for Microbiology; [Washington DC]: American Society for Microbiology, [2018]- (ISSN 2576-098X)
- Published
- 2019
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)