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Can I read TGF-β and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro on EtoBox?
TGF-β and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro by Ilse M.S.L Khouw; Pauline B van Wachem; Josée A Plantinga; Zeljko Vujaskovic; Marcel J.B. Wissink; Lou F.M.H de Leij; Marja J.A van Luyn is a Engineering article available to read on EtoBox.
What is TGF-β and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro about?
Fibroblasts and myo"broblasts are involved in the foreign body reaction to biomaterials, especially in capsule formation. However, contraction or detachment of the capsule can lead to complications. Biocompatibility of biomaterials may be improved by the application of proteins regulating the di!erentiation or activation of (myo)"broblasts. Myo"broblasts, di!erentiating from "broblasts can be identi"ed by the expression of -smooth muscle actin ( -SM actin). We investigated the in#uence of proliferation and quiescence on the di!erentiation of porcine dermal cells and whether transforming growth factor-(TGF-) and basic "broblast growth factor (bFGF) are involved in the di!erentiation of proliferating cells. Porcine cells were used because pigs increasingly function as in vivo models while little is known of the characteristics of their cells. Serum-free cultured, quiescent "broblasts di!erentiated into myo"broblasts, while proliferating "broblasts cultured in the presence of serum containing TGF-, formed -SM actin-negative cell clusters. After reaching con#uency, these clusters started to expressing -SM actin. Moreover, these proliferating cells produced TGF-from day 4 onwards while
Who reads TGF-β and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Ilse M.S.L Khouw; Pauline B van Wachem; Josée A Plantinga; Zeljko Vujaskovic; Marcel J.B. Wissink; Lou F.M.H de Leij; Marja J.A van Luyn
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 1999
- Language
- EN
- Field
- Engineering (Physical Sciences)