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MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells by D'Onofrio, Nunzia (author);Prattichizzo, Francesco (author);Martino, Elisa (author);Anastasio, Camilla (author);Mele, Luigi (author);La Grotta, Rosalba (author);Sardu, Celestino (author);Ceriello, Antonio (author);Marfella, Raffaele (author);Paolisso, Giuseppe (author);Balestrieri, Maria Luisa (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells about?
MiR-27b is highly expressed in endothelial cells (EC) but its function in this context is poorly characterized. This study aims to investigate the effect of miR-27b on inflammatory pathways, cell cycle, apoptosis, and mitochondrial oxidative imbalances in immortalized human aortic endothelial cells (teloHAEC), human umbilical vein endothelial cells (HUVEC), and human coronary artery endothelial cells (HCAEC) exposed to TNF-α. Treatment with TNF-α downregulates the expression of miR-27b in all EC lines, promotes the activation of inflammatory pathways, induces mitochondrial alteration and reactive oxygen species accumulation, fostering the induction of intrinsic apoptosis. Moreover, miR-27b mimic counteracts the TNF-α-related cytotoxicity and inflammation, as well as cell cycle arrest and caspase-3-dependent apoptosis, restoring mitochondria redox state, function, and membrane polarization. Mechanistically, hsa-miR-27b-3p targets the 3′untranslated regions of FOXO1 mRNA to downregulate its expression, blunting the activation of the Akt/FOXO1 pathway. Here, we show that miR-27b is involved in the regulation of a broad range of functionally intertwined phenomena in EC, suggesting its
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It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- D'Onofrio, Nunzia (author);Prattichizzo, Francesco (author);Martino, Elisa (author);Anastasio, Camilla (author);Mele, Luigi (author);La Grotta, Rosalba (author);Sardu, Celestino (author);Ceriello, Antonio (author);Marfella, Raffaele (author);Paolisso, Giuseppe (author);Balestrieri, Maria Luisa (author)
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)