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Association between reduced levels of MEFV messenger RNA in peripheral blood leukocytes and acute inflammation by Duran Üstek; Cumhur Gökhan Ekmekci; Fatih Selçukbiricik; Aris Cakiris; Basar Oku; Burcak Vural; Hakan Yanar; Korhan Taviloglu; Ugur Özbek; Ahmet Gül is a Medicine article available to read on EtoBox.

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## Abstract ## Objective Familial Mediterranean fever (FMF) is associated with more than 70 missense mutations in the __MEFV__ gene. The purpose of this study was to investigate the relative expression of messenger RNA (mRNA) for the __MEFV__ gene in peripheral blood leukocytes (PBLs) obtained from patients with FMF during attacks of acute abdominal inflammation as well as during asymptomatic periods. ## Methods We studied 16 patients with FMF during an attack of acute peritonitis and 17 otherwise healthy individuals who were undergoing surgery because of acute appendicitis. Blood samples were collected from both groups of patients during both acute inflammatory and asymptomatic periods. Relative levels of __MEFV__ mRNA in PBLs were detected with real‐time reverse transcriptase–polymerase chain reaction using LightCycler, with 2 sets of primers for the __MEFV__ gene (exons 7–10 and exons 2–3) and with primers for __CIAS1__ and __PSTPIP1__ genes. Expression levels were compared with β~2~‐microglobulin as an internal control. ## Results __MEFV__ expression was reduced in FMF patients during asymptomatic periods as compared with the non‐FMF controls (__P__ < 0.001). We observed a furt

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Author

Duran Üstek; Cumhur Gökhan Ekmekci; Fatih Selçukbiricik; Aris Cakiris; Basar Oku; Burcak Vural; Hakan Yanar; Korhan Taviloglu; Ugur Özbek; Ahmet Gül

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0004-3591)

Published

2007

Language

EN

Field

Medicine (Health Sciences)