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Can I read Firming of fruit tissues by vacuum-infusion of pectin methylesterase: Visualisation of enzyme action on EtoBox?
Firming of fruit tissues by vacuum-infusion of pectin methylesterase: Visualisation of enzyme action by Anne Guillemin; Fabienne Guillon; Pascal Degraeve; Corinne Rondeau; Marie-Françoise Devaux; Françoise Huber; Eric Badel; Rémi Saurel; Marc Lahaye is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Firming of fruit tissues by vacuum-infusion of pectin methylesterase: Visualisation of enzyme action about?
Apple pieces were vacuum-impregnated with either a pectin methylesterase (PME) and calcium solution or with water prior to pasteurization. Pasteurized apple pieces impregnated with PME and calcium showed a significantly higher firmness. Moreover, solid state (13)C NMR spectroscopy of apple cell wall residues revealed an increase of their molecular rigidity. Exogenous PME addition involved a decrease from 82% to 45% of apple pectin degree of methyl-esterification. Microscopic observations of apple slices immunolabelled with antibodies specific for pectins showed that (i) demethyl-esterification was more intense in the cell wall region lining intercellular spaces (demonstrating a key role for these intercellular channels in the enzyme penetration in the tissue during vacuum-infusion) and that (ii) the number of calcium-dimerized deesterified homogalacturonan chains increased. The results corroborate the hypothesis that vacuum-impregnated PME action liberates free carboxyl groups along pectin chains that could interact with calcium, increasing the rigidity of pectins and finally the mechanical rigidity of apple tissue.
Who reads Firming of fruit tissues by vacuum-infusion of pectin methylesterase: Visualisation of enzyme action?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Anne Guillemin; Fabienne Guillon; Pascal Degraeve; Corinne Rondeau; Marie-Françoise Devaux; Françoise Huber; Eric Badel; Rémi Saurel; Marc Lahaye
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0308-8146)
- Published
- 2008
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)