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Hydrogen production by continuous cultures of Escherchia coli under different nutrient regimes by Jonathan Turcot; Ariane Bisaillon; Patrick C. Hallenbeck is a Engineering article available to read on EtoBox.

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The effects of nutrient limitations on hydrogen yields during fermentation by chemostat cultures of Escherchia coli were studied. Four nutrient regimes (limiting nutrients) were chosen; glucose, nitrogen, sulfur and phosphate. Yields with limiting phosphate or sulfate were low, at or below 1 mol H 2 =mol glucose. Yields with continuous cultures limited for nitrogen decreased with increasing dilution rate ðDÞ, and were lower than that obtained with a static culture ðD 1⁄4 0 h À1 Þ. The highest yields (at, or somewhat higher than 2 mol H 2 =mol glucose) were obtained with cultures limited for glucose. Under most conditions, total hydrogen production and yield were inversely related, with higher production, but lower yields, at increasing dilution rate. A strain, DJT135, mutated in the uptake hydrogenases, in lactate dehydrogenase, and with constitutively active FhlA gave the highest H 2 production and greatest H 2 to glucose yields.

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Author

Jonathan Turcot; Ariane Bisaillon; Patrick C. Hallenbeck

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0360-3199)

Published

2008

Language

EN

Field

Engineering (Physical Sciences)