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Pex3p‐dependent Peroxisomal Biogenesis Initiates in the Endoplasmic Reticulum of Human Fibroblasts by Andrés A. Toro; Claudia A. Araya; Gonzalo J. Córdova; Cristian A. Arredondo; Hugo G. Cárdenas; Regina E. Moreno; Alejandro Venegas; Cecilia S. Koenig; Jorge Cancino; Alfonso Gonzalez; Manuel J. Santos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Pex3p‐dependent Peroxisomal Biogenesis Initiates in the Endoplasmic Reticulum of Human Fibroblasts about?
## Abstract The mechanisms of peroxisomal biogenesis remain incompletely understood, specially regarding the role of the endoplasmic reticulum (ER) in human cells, where genetic disorders of peroxisome biogenesis lead to Zellweger syndrome (ZS). The Pex3p peroxisomal membrane protein (PMP) required for early steps of peroxisome biogenesis has been detected in the ER in yeast but not in mammalian cells. Here, we show that Pex3p‐GFP expressed in a new ZS cell line (MR), which lacks peroxisomes due to a mutation in the PEX3 gene, localizes first in the ER and subsequently in newly formed peroxisomes. Pex3p bearing an artificial __N__‐glycosylation site shows an electrophoretic shift indicative of ER targeting while en route to preformed peroxisomes in normal fibroblast. A signal peptide that forces its entry into the ER does not eliminate its capability to drive peroxisome biogenesis in ZS cells. Thus, Pex3p is able to drive peroxisome biogenesis from the ER and its ER pathway is not privative of ZS cells. Cross‐expression experiments of Pex3p in GM623 cells lacking Pex16p or Pex16p in MR cells lacking Pex3p, showed evidence that Pex3p requires Pex16p for ER location but is dispensabl
Who reads Pex3p‐dependent Peroxisomal Biogenesis Initiates in the Endoplasmic Reticulum of Human Fibroblasts?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Andrés A. Toro; Claudia A. Araya; Gonzalo J. Córdova; Cristian A. Arredondo; Hugo G. Cárdenas; Regina E. Moreno; Alejandro Venegas; Cecilia S. Koenig; Jorge Cancino; Alfonso Gonzalez; Manuel J. Santos
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)