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This document describes a real-time PCR method for quantifying the amount of bovine and buffalo DNA in dairy and meat products. The method uses species-specific primers and probes targeting bovine and buffalo mitochondrial DNA. Calibration curves generated from controlled mixtures of bovine and buffalo reference materials are used to normalize DNA quantities between species and food matrices. Testing on dairy samples with known bovine and buffalo content showed the method accurately approximated measured qu

Author

MihaelaOlaru

Language

EN