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Rapid and simple detection of IFN-neutralizing antibodies in chronic hepatitis C non-responsive to IFN-α by Carl Jorns; Dirk Holzinger; Robert Thimme; Hans Christian Spangenberg; Manfred Weidmann; Jens Rasenack; Hubert Erich Blum; Otto Haller; Georg Kochs is a Medicine article available to read on EtoBox.
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## Abstract Different mechanisms have been proposed for the failure of interferon (IFN) therapy in patients with chronic hepatitis C and multiple sclerosis, for example, the presence of IFN‐neutralizing antibodies. In this study, a novel assay system based on the IFN‐inducible __Mx__‐promoter was used to detect IFN‐neutralizing antibodies in sera of patients with chronic hepatitis C. To monitor IFN bioactivity in IFN‐treated patients, a real‐time RT‐PCR for __MxA__ gene expression in PBMCs was established. Using these two methods, patients with chronic hepatitis C virus (HCV) infection receiving IFN therapy and patients with treatment induced HCV clearance were monitored. Importantly, neutralizing anti‐IFN antibodies were detected in the sera of 3 of 38 chronically HCV‐infected patients who failed to respond to therapy but none in sera of patients who cleared HCV after IFN therapy. Interestingly, the presence of these antibodies correlated with the lack of __MxA__ induction in PBMCs after initiation of IFN‐α therapy. Retrospective analysis of one patient's sera revealed that the anti‐IFN‐α antibodies had already developed after the first of four unsuccessful IFN therapies, suggesti
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- Author
- Carl Jorns; Dirk Holzinger; Robert Thimme; Hans Christian Spangenberg; Manfred Weidmann; Jens Rasenack; Hubert Erich Blum; Otto Haller; Georg Kochs
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)