About this Medicine article

Evidence for the Critical Role of Interleukin‐12 but Not Interferon‐γ in the Pathogenesis of Experimental Colitis in Mice by KOTARO TOZAWA; HIROYUKI HANAI; KEN SUGIMOTO; SATOSHI BABA; HARUHIKO SUGIMURA; TAIKI AOSHI; MASATO UCHIJIMA; TOSHI NAGATA; YUKIO KOIDE is a Medicine article available to read on EtoBox.

## Abstract **Background and Aims:** The imbalance between helper T (Th)1/Th2 cytokines has been observed in human inflammatory bowel disease and various animal models. Because interleukin (IL)‐12 and interferon‐γ (IFN‐γ) productions are known to be a hallmark of Th1‐dominant intestinal inflammation such as 2,4,6‐trinitrobenzene sulfonic acid (TNBS)‐induced colitis, we strictly addressed the roles of IFN‐γ and IL‐12 in the development of colitis, employing knockout mice with IFN‐γ receptor (IFN‐γR) or IL‐12 p40 gene disruptions and mice administered with neutralizing monoclonal antibodies (mAbs) against IFN‐γ or IL‐12. **Methods:** To induce colitis, 2.5 mg of the hapten reagent TNBS in 50% ethanol was administered into the colon. Two milligrams of rat anti‐mouse IFN‐γ mAb, rat anti‐mouse IL‐12 mAb, or control rat IgG were administered intraperitoneally into mice before TNBS administration. Change in the body weight of mice was evaluated and the degree of inflammation of the colon of these mice was investigated histologically. Immunohistochemical and immunofluorescence analyses were performed to detect CD4^+^ T cells, macrophages and IL‐12 in TNBS‐induced colitis lesions. The profi

Author

KOTARO TOZAWA; HIROYUKI HANAI; KEN SUGIMOTO; SATOSHI BABA; HARUHIKO SUGIMURA; TAIKI AOSHI; MASATO UCHIJIMA; TOSHI NAGATA; YUKIO KOIDE

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0815-9319)

Published

2003

Language

EN

Field

Medicine (Health Sciences)