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Role of the periplasmic domain of the Escherichia coli NarX sensor-transmitter protein in nitrate-dependent signal transduction and gene regulation by Ricardo Cavicchioli; Robin C. Chiang; Lisa V. Kalman; Robert P. Gunsalus is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Role of the periplasmic domain of the Escherichia coli NarX sensor-transmitter protein in nitrate-dependent signal transduction and gene regulation about?

The __narX__, __narQ__ and __narL__ genes of __Escherichia coli__ encode a nitrate‐responsive two‐component regulatory system that controls the expression of many anaerobic electron‐transport‐ and fermentation‐related genes. When nitrate is present, the NarX and NarQ sensor‐transmitter proteins function to activate the response‐regulator protein, NarL, which in turn binds to its DNA‐recognition sites to modulate gene expression. The sensor‐transmitter proteins are anchored in the cytoplasmic membrane by two transmembrane domains that are separated by a periplasmic region of ≈115 amino acids. In this study we report the isolation and characterization of __narX__\* (star) mutants that constitutively activate nitrate reductase (__narGHJI__) gene expression and repress fumarate reductase (__frdABCD__) gene expression when no nitrate is provided for the cell. An additional __narX__ mutant was identified that has lost its ability to respond to environmental signals. Each __narX__ defect was caused by a single amino acid substitution within a conserved 17 amino acid sequence, called the ‘P‐box’, in the periplasmic exposed region of the NarX protein. As a result, DNA binding is then ‘locke

Who reads Role of the periplasmic domain of the Escherichia coli NarX sensor-transmitter protein in nitrate-dependent signal transduction and gene regulation?

Author

Ricardo Cavicchioli; Robin C. Chiang; Lisa V. Kalman; Robert P. Gunsalus

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)