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Lactobacillus casei is grown under anaerobic conditions in a 40,000 L fed-batch fermenter operated at quasi steady-state over 6 hours with a feed rate of 4,000 L/hr and substrate concentration in the feed of 80 g/L. The initial culture volume is calculated to be 16,000 L. The substrate concentration at quasi steady-state after 6 hours is calculated to be 0.06 g/L. The cell concentration at quasi steady-state is calculated to be 18.39 g/L. The total mass of cells produced after 6 hours is calculated to be

Author

Trường Tùng

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EN