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Meselson and Stahl used isotopic labeling to confirm that DNA replication is semiconservative. They grew E. coli in media containing heavy nitrogen isotopes, producing labeled "heavy" DNA. After replication, they found DNA existed in two bands - one heavy and one intermediate - showing that the parental strands were conserved in daughter molecules but each daughter contained one old and one new strand. This supported Watson and Crick

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MIM242

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EN