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Staphylococcus aureus Cowan Strain 1 Activation of B-Chronic Lymphocytic Leukaemia Cells Augments the Response to CD40 Stimulation by Söderberg; Thunberg; Weigelt; Christiansen; Tötterman; Carlsson; Sällström; Nilsson is a Immunology and Microbiology article available to read on EtoBox.

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The signals involved in regulating the proliferation, differentiation and survival of B‐chronic lymphocytic leukemia (B‐CLL) cells are fully understood. B‐CLL cells have been found to respond poorly to various activation signals and only after successful Epstein–Barr virus (EBV) transformation has it been possible to maintain such cells in long‐term cultures. In this work we describe a new method to activate and induce proliferation in B‐CLL cells and to maintain such cells in long‐term culture for longer than 1 month. We used a combination of protocols in an attempt to mimic some of the signals of a thymus‐dependent immune response. The B‐CLL cells were first activated with __Staphylococcus aureus__ Cowan strain 1 (SAC) particles plus thioredoxin (Trx), followed by stimulation with interleukin (IL)‐2 + Trx. This treatment primed the cells for further stimulation with anti‐CD40 monoclonal antibody (MoAb) presented on irradiated CD32L cells (the CD40‐system) or soluble CD40 Ligand, and a combination of Trx and cytokines (IL‐4 + IL‐10), which allowed the cells to be maintained for up to 1 month with preserved viability and a variable rate of proliferation. However, induced proliferat

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Author

Söderberg; Thunberg; Weigelt; Christiansen; Tötterman; Carlsson; Sällström; Nilsson

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0300-9475)

Published

1999

Language

EN

Field

Immunology and Microbiology (Life Sciences)