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Efficient Synthesis of Oligonucleotide Conjugates on Solid-support Using an (Aminoethoxycarbonyl)aminohexyl Group for 5′-Terminal Modification by Naoshi Kojima; Toshie Takebayashi; Akiko Mikami; Eiko Ohtsuka; Yasuo Komatsu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Solid-support conjugation at the 5 0 -terminal primary amine of oligonucleotides is a convenient and powerful method for introducing various functional groups. However, conventional aliphatic amines do not necessarily provide conjugates with sufficient yields. To improve the modification efficacy, we used the amino-linker (aminoethoxycarbonyl)aminohexyl group (ssH-linker), for solid-support conjugation. In the ssH-linker terminal modification, reactive free amino group could be easily presented onto a solid-support due to rapid removal of the amino-protecting group, and activated amino acids or cholesterol molecules could be covalently connected more efficiently than to typical 6-aminohexyl-linkers. Based on these results, the ssH-linker can be a useful terminal modification for the solid-support conjugation of functional molecules.

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Author

Naoshi Kojima; Toshie Takebayashi; Akiko Mikami; Eiko Ohtsuka; Yasuo Komatsu

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0960-894X)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)