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Phenotype, genotype and clonality of Reed-Sternberg cells in nodular sclerosis Hodgkin’s disease: results of a single-cell study by Jan D elabie; Anne Tierens; Theodora Gavriil; Gang Wu; Dennis D. Weisenburger; Wing C. Chan is a Medicine article available to read on EtoBox.

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The genotype and clonality of Reed‐Sternberg (RS) cells in Hodgkin’s disease (HD) has remained a controversial issue, largely due to the scarcity of RS cells in tissues and the limitations of the techniques used to resolve this issue. Southern hybridization and polymerase chain reaction (PCR) assays using DNA extracted from tissues can only document clonal gene rearrangements, but do not indicate which cellular population is responsible for such rearrangements. To overcome the limitations of these previous techniques for studying the genotype and clonality of RS cells, we analysed single RS cells with a single‐cell PCR assay to detect immunoglobulin heavy chain gene (IgH) rearrangements and X‐chromosome inactivation. Six cases of nodular sclerosis (NS) HD were studied. The RS cells displayed a B‐cell phenotype in three cases and a null‐cell phenotype in the other three cases. IgH rearrangements were detected in the RS cells of the three cases with a B‐cell phenotype, but not in the other cases. In these three cases the IgH rearrangements in the RS cells were polyclonal, although a subpopulation of clonal RS cells was documented in one case. The finding that the RS cells with IgH re

Who reads Phenotype, genotype and clonality of Reed-Sternberg cells in nodular sclerosis Hodgkin’s disease: results of a single-cell study?

Author

Jan D elabie; Anne Tierens; Theodora Gavriil; Gang Wu; Dennis D. Weisenburger; Wing C. Chan

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

1996

Language

EN

Field

Medicine (Health Sciences)