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Can I read Sialic Acid in Human Serum and Cerebrospinal Fluid. Comparison of methods and reference values on EtoBox?
Sialic Acid in Human Serum and Cerebrospinal Fluid. Comparison of methods and reference values by K. Lorentz; T. Weiß; E. Kraas is a Medicine article available to read on EtoBox.
What is Sialic Acid in Human Serum and Cerebrospinal Fluid. Comparison of methods and reference values about?
Sialic acid was estimated simultaneously by three methods: (A) chemical determination based on Warrerfs method (Meth. Enzymol. 6, 463-464 (1963)) with slight modifications, (B) enzymatic measurement with a commercially available test kit, and (C) high performance liquid chromatography (HPLC) according to Silver et al. (J. Chromatogr. 224, 381-388(1981)). These methods showed closely correlated (r > 0.930) results and displayed similar precision data. Interference studies demonstrated sufficient specificity for the chemical assay, which was 5 -6 times more sensitive than the enzymatic test and hence chosen for the establishment of reference values. From 249 sera from healthy people between 16 and 63 years the 0.025 -0.975-reference intervals were calculated to be 1.57-2.63 mmol/1 for 127 men, and 1.69-2.64 mmol/1 for 122 women with no significant dependence on age and sex. From 43 cerebrospinal fluids from healthy adults the respective values were 17. 3 -50.4 / . These data correspond to those of the literatüre. Some chemical assays employing thiobarbituric acid were compared. They proved reliable in contrast to the reaction of serum with 4-dimethylaminobenzaldehyde. Sialinsäure im
Who reads Sialic Acid in Human Serum and Cerebrospinal Fluid. Comparison of methods and reference values?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- K. Lorentz; T. Weiß; E. Kraas
- Published
- 1986
- Language
- EN
- Field
- Medicine (Health Sciences)