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Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells by Rooda, Ilmatar; Hensen, Kati; Kaselt, Birgitta; Kasvandik, Sergo; Pook, Martin; Kurg, Ants; Salumets, Andres; Velthut-Meikas, Agne is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells about?

MicroRNAs (miRNAs) are known post-transcriptional regulators of various biological processes including ovarian follicle development. We have previously identified miRNAs from human pre-ovulatory ovarian granulosa cells that are expressed from the intronic regions of two key genes in normal follicular development: FSH receptor (__FSHR__) and __CYP19A1__, the latter encoding the aromatase enzyme. The present study aims to identify the target genes regulated by these miRNAs: hsa-miR-548ba and hsa-miR-7973, respectively. The miRNAs of interest were transfected into KGN cell line and the gene expression changes were analyzed by Affymetrix microarray. Potential miRNA-regulated genes were further filtered by bioinformatic target prediction algorithms and validated for direct miRNA:mRNA binding by luciferase reporter assay. __LIFR__, __PTEN__, __NEO1__ and __SP110__ were confirmed as targets for hsa-miR-548ba. Hsa-miR-7973 target genes __ADAM19__, __PXDN__ and __FMNL3__ also passed all verification steps. Additionally, the expression pattern of the miRNAs was studied in human primary cumulus granulosa cell culture in relation to the expression of their host genes and FSH stimulation. Based

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Author

Rooda, Ilmatar; Hensen, Kati; Kaselt, Birgitta; Kasvandik, Sergo; Pook, Martin; Kurg, Ants; Salumets, Andres; Velthut-Meikas, Agne

Publisher

Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)