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Scalable Production of Adeno‐associated Virus Type 2 Vectors via Suspension Transfection by Joon Young Park; Byung‐Pil Lim; Kyuhyun Lee; Young‐Gun Kim; Eui‐Cheol Jo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Vectors derived from adeno‐associated virus type 2 (AAV2) are promising gene delivery vehicles, but it is still challenging to get the large number of recombinant adeno‐associated virus (rAAV) particles required for large animal and clinical studies. Current transfection technology requires adherent cultures of HEK 293 cells that can only be expanded by preparing multiple culture plates. A single large‐scale suspension culture could replace these multiple culture preparations, but there is currently no effective co‐transfection scheme for generating rAAV from cells in suspension culture. Here, we weaned HEK 293 cells to suspension culture using hydrogel‐coated six‐well culture plates and established an efficient transfection strategy suitable for these cells. Then the cultures were gradually scaled up. We used linear polyethylenimine (PEI) to mediate transfection and obtained high transfection efficiencies ranging from 54% to 99%, thereby allowing efficient generation of rAAV vectors. Up to 10^13^ rAAV particles and, more importantly, up to 10^11^ infectious particles were generated from a 2‐L bioreactor culture. The suspension‐transfection strategy of this study facili

Who reads Scalable Production of Adeno‐associated Virus Type 2 Vectors via Suspension Transfection?

Author

Joon Young Park; Byung‐Pil Lim; Kyuhyun Lee; Young‐Gun Kim; Eui‐Cheol Jo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)