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Carica papaya latex is a rich source of a class II chitinase by Mohamed Azarkan; Amina Amrani; Michelle Nijs; André Vandermeers; Samira Zerhouni; Nicole Smolders; Yvan Looze is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Carica papaya latex is a rich source of a class II chitinase about?
A class II chitinase is present in the latex of the tropical species Carica papaya. The enzyme may be readily purified by using a combination of hydrophobic interaction-and cation-exchange chromatography. This enzyme preparation is homogeneous with respect to the three physico-chemical criteria of charge, Mr (28 000) and hydrophobicity. It is also completely free of any proteolytic and bacteriolytic activities. The enzyme was classified as a class II chitinase on the basis of its N-terminal amino acid sequence up to the 30th residue. In agreement with this classification, the enzyme preparation hydrolyses chitinase substrates only very slowly and several free thiol functions are present in the polypeptide chain. These free thiol functions are buried, and to be available for titration with 2,2'-dipyridyldisulphide, the enzyme must be denatured. Unfolding of papaya chitinase requires particularly drastic conditions, not less than 4 M guanidinium hydrochloride at 25 ° and pH 6.8.
Who reads Carica papaya latex is a rich source of a class II chitinase?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mohamed Azarkan; Amina Amrani; Michelle Nijs; André Vandermeers; Samira Zerhouni; Nicole Smolders; Yvan Looze
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)
- Published
- 1997
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)