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Plasmid-based Genetic Modification of Human Bone Marrow-derived Stromal Cells: Analysis of Cell Survival and Transgene Expression After Transplantation in Rat Spinal Cord by Mark W Ronsyn; Jasmijn Daans; Gie Spaepen; Shyama Chatterjee; Katrien Vermeulen; Patrick D'Haese; Viggo FI Van Tendeloo; Eric Van Marck; Dirk Ysebaert; Zwi N Berneman; Philippe G Jorens; Peter Ponsaerts is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Plasmid-based Genetic Modification of Human Bone Marrow-derived Stromal Cells: Analysis of Cell Survival and Transgene Expression After Transplantation in Rat Spinal Cord about?
## Background Bone marrow-derived stromal cells (MSC) are attractive targets for __ex vivo__ cell and gene therapy. In this context, we investigated the feasibility of a plasmid-based strategy for genetic modification of human (h)MSC with enhanced green fluorescent protein (EGFP) and neurotrophin (NT)3. Three genetically modified hMSC lines (EGFP, NT3, NT3-EGFP) were established and used to study cell survival and transgene expression following transplantation in rat spinal cord. ## Results First, we demonstrate long-term survival of transplanted hMSC-EGFP cells in rat spinal cord under, but not without, appropriate immune suppression. Next, we examined the stability of EGFP or NT3 transgene expression following transplantation of hMSC-EGFP, hMSC-NT3 and hMSC-NT3-EGFP in rat spinal cord. While __in vivo__ EGFP mRNA and protein expression by transplanted hMSC-EGFP cells was readily detectable at different time points post-transplantation, __in vivo__ NT3 mRNA expression by hMSC-NT3 cells and __in vivo__ EGFP protein expression by hMSC-NT3-EGFP cells was, respectively, undetectable or declined rapidly between day 1 and 7 post-transplantation. Further investigation revealed that the o
Who reads Plasmid-based Genetic Modification of Human Bone Marrow-derived Stromal Cells: Analysis of Cell Survival and Transgene Expression After Transplantation in Rat Spinal Cord?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mark W Ronsyn; Jasmijn Daans; Gie Spaepen; Shyama Chatterjee; Katrien Vermeulen; Patrick D'Haese; Viggo FI Van Tendeloo; Eric Van Marck; Dirk Ysebaert; Zwi N Berneman; Philippe G Jorens; Peter Ponsaerts
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6750)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)