About this Medicine article

The Expression of Osteoclastogenesis-associated Factors and Osteoblast Response to Osteolytic Prostate Cancer Cells by Colm Morrissey; Janice S. Lai; Lisha G. Brown; Ya-Chun Wang; Martine P. Roudier; Ilsa M. Coleman; Roman Gulati; Funda Vakar-Lopez; Lawrence D. True; Eva Corey; Peter S. Nelson; Robert L. Vessella is a Medicine article available to read on EtoBox.

## Abstract ## BACKGROUND Prostate cancer (PCa) has a propensity to metastasize to bone. Tumor cells replace bone marrow and can elicit an osteoblastic, osteolytic, or mixed bone response. Our objective was to elucidate the mechanisms and key factors involved in promoting osteoclastogenesis in PCa bone metastasis. ## METHODS We cultured osteoblast‐like MC3T3‐E1 cells with conditioned medium (CM) from PC‐3 and C4‐2B cells. MC3T3‐E1 mineralization decreased in the presence of PC‐3 CM, whereas C4‐2B CM had no effect on mineralization. Using oligo arrays and validating by real‐time PCR, we observed a decrease in the expression of mineralization‐associated genes in MC3T3‐E1 cells grown in the presence of PC‐3 CM. In addition, PC‐3 CM induced the expression of osteoclastogenesis‐associated genes IGFBP‐5, IL‐6, MCP‐1, and RANKL while decreasing OPG expression in MC3T3‐E1 cells. Furthermore, CM from MC3T3‐E1 cells cultured in the presence of PC‐3 CM, in association with soluble RANKL, increased osteoclastogenesis in RAW 264.7 cells. Investigation of PCa metastases and xenografts by immunohistochemistry revealed that the osteoclastic factor IL‐6 was expressed in the majority of PCa bone met

Author

Colm Morrissey; Janice S. Lai; Lisha G. Brown; Ya-Chun Wang; Martine P. Roudier; Ilsa M. Coleman; Roman Gulati; Funda Vakar-Lopez; Lawrence D. True; Eva Corey; Peter S. Nelson; Robert L. Vessella

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0270-4137)

Published

2009

Language

EN

Field

Medicine (Health Sciences)