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Structure of the Human Arylsulfatase B Gene by Said MODARESSI; Katrin RUPP; Kurt VON FIGURA; Christoph PETERS is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Structure of the Human Arylsulfatase B Gene about?

We have isolated -phage clones containing the human arylsulfatase B gene region from a genomic X47.1 library. The human arylsulfatase B gene comprises 8 exons interrupted by 7 introns. DN A sequences of all intron-exon boundaries and the 5' flanking region of the gene were determined. All intron-exon splice junctions conformed to the GT/AG consensus sequence. Primer extension analysis revealed multiple start sites 1 to 135 nucleotides 5' of the ATG translational start codon. A 398 bp DNAfragment of the 5 ; flanking region exhibits promotor activity when transiently expressed in BHK-21 cells using the bacterial chloramphenicol acetyltransferase gene as a reporter gene. This putative promotor region is located in a CpG island and contains potential Spl and AP2 binding sites but lacks typical TATA and CAATbox motifs. Key terms: Human arylsulfatase B gene, exon-intron

Who reads Structure of the Human Arylsulfatase B Gene?

Author

Said MODARESSI; Katrin RUPP; Kurt VON FIGURA; Christoph PETERS

Publisher

Walter de Gruyter GmbH

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)