Can I read Glutamate Neurotoxicity in Cortical Cell Culture on EtoBox?

Glutamate Neurotoxicity in Cortical Cell Culture by DW Choi; M Maulucci-Gedde; AR Kriegstein is a Neuroscience article available to read on EtoBox.

What is Glutamate Neurotoxicity in Cortical Cell Culture about?

The central neurotoxicity of the excitatory amino acid neurotransmitter glutamate has been postulated to participate in the pathogenesis of the neuronal cell loss associated with several neurological disease states, but the complexity of the intact nervous system has impeded detailed analysis of the phenomenon. In the present study, glutamate neurotoxicity was studied with novel precision in dissociated cell cultures prepared from the fetal mouse neocortex.Brief exposure to glutamate was found to produce morphological changes in mature cortical neurons beginning as quickly as 90 set after exposure, followed by widespread neuronal degeneration over the next hours. Quantitative dose-toxicity study suggested an ED, of 50-l 00 MM for a 5 min exposure to glutamate. Immature cortical neurons and glia were not injured by such exposures to glutamate. Uptake processes probably do not limit GNT in culture, as the uptake inhibitor dihydrokainate did not potentiate GNT. Possibly reflecting the lack of uptake limitation, glutamate was found to be actually more potent than kainate as a neurotoxin in these cultures, a dramatic reversal of the in vivo potency rank order. Some neurons regularly sur

Who reads Glutamate Neurotoxicity in Cortical Cell Culture?

Author

DW Choi; M Maulucci-Gedde; AR Kriegstein

Published

1987

Language

EN

Field

Neuroscience (Life Sciences)