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Can I read Integrative Modeling of a Sin3/HDAC Complex Sub-structure on EtoBox?
Integrative Modeling of a Sin3/HDAC Complex Sub-structure by Charles A.S. Banks; Ying Zhang; Sayem Miah; Yan Hao; Mark K. Adams; Zhihui Wen; Janet L. Thornton; Laurence Florens; Michael P. Washburn is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Integrative Modeling of a Sin3/HDAC Complex Sub-structure about?
## SUMMARY Sin3/HDAC complexes function by deacetylating histones, condensing chromatin, and modulating gene expression. Although components used to build these complexes have been well defined, we still have only a limited understanding of the structure of the Sin3/HDAC subunits assembled around the scaffolding protein SIN3A. To characterize the spatial arrangement of Sin3 subunits, we combined Halo affinity capture, chemical crosslinking, and high-resolution mass spectrometry (XL-MS) to determine intersubunit distance constraints, identifying 66 interprotein and 63 self-crosslinks for 13 Sin3 subunits. Having assessed crosslink authenticity by mapping self-crosslinks onto existing structures, we used distance restraints from interprotein crosslinks to guide assembly of a Sin3 complex substructure. We identified the relative positions of subunits SAP30L, HDAC1, SUDS3, HDAC2, and ING1 around the SIN3A scaffold. The architecture of this subassembly suggests that multiple factors have space to assemble to collectively influence the behavior of the catalytic subunit HDAC1.
Who reads Integrative Modeling of a Sin3/HDAC Complex Sub-structure?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Charles A.S. Banks; Ying Zhang; Sayem Miah; Yan Hao; Mark K. Adams; Zhihui Wen; Janet L. Thornton; Laurence Florens; Michael P. Washburn
- Publisher
- Elsevier BV
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)