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Expansion Microscopy for Imaging the Cell-material Interface by Melissa L. Nakamoto; Csaba Forro; Wei Zhang; Ching-Ting Tsai; Bianxiao Cui is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Expansion Microscopy for Imaging the Cell-material Interface about?

The cellular environment is dense and complex, and imaging with 3D singlemolecule super-resolution microscopy requires careful consideration of appropriate illumination methods to use in relation to the goals of the study. There is, however, typically a tradeoff between performance and simplicity when selecting illumination schemes. Epi-illumination is a common and simple technique, but it generates high unwanted background fluorescence and causes photodamage and photobleaching throughout the imaged cell. Total internal reflection fluorescence (TIRF) is another technique which provides excellent contrast, but it is limited to imaging a thin volume at the bottom of a sample and is thus incompatible with whole-cell imaging. Light sheet illumination is an alternative, whole-cell compatible approach where a thin sheet of light is directed orthogonally to the detection axis to optically section a cell and reduce the fluorescence background, photobleaching, and photodamage. In this work we demonstrate a versatile microscopy platform which integrates the sectioning and background reducing capability of light sheet illumination with homogeneous, flat-field epi-and TIRF illumination. A flat

Who reads Expansion Microscopy for Imaging the Cell-material Interface?

Author

Melissa L. Nakamoto; Csaba Forro; Wei Zhang; Ching-Ting Tsai; Bianxiao Cui

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)