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The Raman detection of peptide tyrosine phosphorylation by Yong Xie; Dongmao Zhang; Gotam K. Jarori; V.Jo Davisson; Dor Ben-Amotz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The Raman detection of peptide tyrosine phosphorylation about?

Drop-coating-deposition-Raman (DCDR) is used to detect spectral changes induced by phosphorylation of tyrosine amino acid residues in peptides. Four peptides are investigated, with sequences derived from the human protein-tyrosine kinase, p60c-src, with Y-216, Y-419, and Y-530 phosphorylation sites. Although the spectra of the four peptides are quite different, tyrosine phosphorylation is found to invariably induce the collapse of a doublet at 820-850cm(-1) and the attenuation of a peak around 1205cm(-1). Moreover, amide III band shifts suggest that tyrosine phosphorylation may promote beta sheet formation, particularly in peptides that lack phenylalanine residues. The degree of tyrosine phosphorylation in peptide mixtures is determined using DCDR combined with partial least squares multivariate calibration with a 2% root mean standard error of prediction.

Who reads The Raman detection of peptide tyrosine phosphorylation?

Author

Yong Xie; Dongmao Zhang; Gotam K. Jarori; V.Jo Davisson; Dor Ben-Amotz

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)