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Integration of Ileum Cannulated Pigs Andin Vitro Fermentation to Quantify the Effect of Diet Composition on the Amount of Short-chain Fatty Acids Available from Fermentation in the Large Intestine by Christensen, Dorthe N; Knudsen, Knud Erik Bach; Wolstrup, Jens; Jensen, Bent Borg is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Integration of Ileum Cannulated Pigs Andin Vitro Fermentation to Quantify the Effect of Diet Composition on the Amount of Short-chain Fatty Acids Available from Fermentation in the Large Intestine about?

Using cannulated pigs and a standardised in vitro fermentation system the eþ ect of diet and non-starch polysaccharides (NSP) on the amount of energy available from microbial fermentation in the large intestine could be predicted. The available energy was calculated from the amounts of shortchain fatty acids (SCFA) produced. Three diets were investigated : a low übre diet based essentially on wheat ýour (56 g NSP kg-1 feed) and two high übre diets with added oat bran (93 g NSP kg-1 feed) or wheat bran (102 g NSP kg-1 feed). Colonic fermentation was estimated by in vitro fermentation of freeze-dried ileal effluent collected from cannulated pigs. The in vitro fermentation method was optimised to use 10 g ileum content litre-1 incubated at pH 6.0 in a fermentor containing faecal slurry consisting of anaerobic mineral salts medium and 50 g litre-1 faeces from pigs fed the same diets as the cannulated pigs. The results demonstrate that it is very important to compensate for the faecal SCFA contribution when calculating the amount of SCFA produced from ileal digesta during in vitro fermentation. The amount of NSP digested in vitro was compared with data obtained from in vivo studies and

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Author

Christensen, Dorthe N; Knudsen, Knud Erik Bach; Wolstrup, Jens; Jensen, Bent Borg

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-5142)

Published

1999

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)