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Chromatofocusing Fractionation and Two-dimensional Difference Gel Electrophoresis for Low Abundance Serum Proteins by Shuzhen Qin; Angeline S. Ferdinand; Jerome P. Richie; Michael P. O'Leary; Samuel C. Mok; Brian C.-S. Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Chromatofocusing Fractionation and Two-dimensional Difference Gel Electrophoresis for Low Abundance Serum Proteins about?
## Abstract The technical challenge to analysis of the serum proteome is that the serum proteins are present at unequal concentrations. A few are so dominant, such as serum albumin and immunoglobulins, that they mask detection of other proteins. Because of these high abundance proteins, current technologies, while theoretically capable of analyzing protein amounts spanning four orders of magnitude, are only able to analyze proteins ranging over two orders of magnitude and cannot analyze the lower abundance proteins that may be the next biomarkers and drug targets. To facilitate the identification of low abundance proteins, we fractionated serum samples from patients with prostate cancer and patients with benign prostate hyperplasia using anion displacement liquid chromatofocusing chromatography, which separates proteins by a pH gradient and a positively charged column. Differential expression of proteins from fractions was then determined and identified by IEF gels and 2‐D DIGE. Results demonstrate improved resolution of proteins within the chosen pH gradient when compared to the unfractionated samples. Several proteins that were differentially expressed in serum from patients with
Who reads Chromatofocusing Fractionation and Two-dimensional Difference Gel Electrophoresis for Low Abundance Serum Proteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Shuzhen Qin; Angeline S. Ferdinand; Jerome P. Richie; Michael P. O'Leary; Samuel C. Mok; Brian C.-S. Liu
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)